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Open Archive Published:June 19, 2014 DOI:https://doi.org/10.1016/j.stem.2014.06.008
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    Highlights

    • LepR + cells account for 0.3% of cells and 94% of CFU-Fs in adult bone marrow
    • LepR + cells form osteoblasts, chondrocytes, and adipocytes in culture and in vivo
    • LepR + cells give rise to most of the bone and adipocytes formed in adult marrow
    • LepR + cells are normally quiescent but proliferate after injury to regenerate bone
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    Summary

    Studies of the identity and physiological function of mesenchymal stromal cells (MSCs) have been hampered by a lack of markers that permit both prospective identification and fate mapping in vivo. We found that Leptin Receptor (LepR) is a marker that highly enriches bone marrow MSCs. Approximately 0.3% of bone marrow cells were LepR +, 10% of which were CFU-Fs, accounting for 94% of bone marrow CFU-Fs. LepR + cells formed bone, cartilage, and adipocytes in culture and upon transplantation in vivo. LepR + cells were Scf-GFP +, Cxcl12-DsRed high, and Nestin-GFP low, markers which also highly enriched CFU-Fs, but negative for Nestin-CreER and NG2-CreER, markers which were unlikely to be found in CFU-Fs. Fate-mapping showed that LepR + cells arose postnatally and gave rise to most bone and adipocytes formed in adult bone marrow, including bone regenerated after irradiation or fracture. LepR + cells were quiescent, but they proliferated after injury. Therefore, LepR + cells are the major source of bone and adipocytes in adult bone marrow.

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    Linked Article

    • Leptin Receptor Makes Its Mark on MSCs
      Cell Stem Cell August 07, 2014
      • Although mesenchymal stem/stromal cells (MSCs) are an important component of the hematopoietic niche, the markers that correlate with their physiological functions have not been defined. In this issue of Cell Stem Cell, Zhou et al. (2014) identify the Leptin Receptor as a marker for prospective identification and in vivo fate mapping of bone marrow MSCs.
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